zebrafish ab strain  (ZIRC Inc)

Journal: EMBO Molecular Medicine

Article Title: FOXK2 in skeletal muscle development: a new pathogenic gene for congenital myopathy with ptosis

doi: 10.1038/s44321-025-00247-x

Figure Lengend Snippet: ( A ) Structure of the zebrafish foxk2 gene. The zebrafish foxk2 gene was targeted using specific MOs to inhibit transcription at the start codon (ATG-MO) and proper splicing of exon 3 (I2E3-MO). ( B ) Quantitative of foxk2 expression level by qPCR. Larvae were collected at 72 hpf ( n = 3 repeats). P value: **** P < 0.0001. ( C ) Percentage of larvae exhibiting defect or mortality. The control-MO group included 379 larvae, the ATG-MO group included 296 larvae, and the I2E3-MO group included 375 larvae. ( D ) Survival rate of larvae over a 72-hpf time course in control-MO, ATG-MO, and I2E3-MO groups. ( E ) Gross morphology of Tg(-1.9mylpfa:EGFP) larvae at 72-hpf, along with the quantification of curvature angle of larvae ( n = 10 larvae for each group). The angle formed between the blue arrows indicates the degree of body curvature in the larvae. Scale bars: 500 µm. P value: **** P < 0.0001. ( F ) Body muscle morphology of Tg(-1.9mylpfa:EGFP) larvae at 72-hpf. Blue and yellow arrows denote normal and defective somites, respectively. The white asterisk indicates an abnormal trunk orientation. The inset in the upper right corner shows a magnified view of the boxed area (scale bars: 10 µm for the inset, 50 µm for the full image). ( G ) Cranial facial muscles of Tg(-1.9mylpfa:EGFP) larvae at 72-hpf. The inset in the upper right corner shows a magnified view of the boxed area (scale bars: 40 µm for the inset, 200 µm for the full image). ( H – K ) Statistical analysis of ( H ) movement distance ( P value: **** P < 0.0001), ( I ) velocity (**** P < 0.0001), ( J ) maximum acceleration (**** P < 0.0001) and ( K ) mobility (percentage of actual moving distance to total distance) (*** P = 0.0001; * P = 0.0231) in larvae at 5-dpf ( n = 10 larvae for each froup). Data were analyzed by Student’s t test. All error bars indicate the mean ± standard deviation. MO morpholino, hpf hours post fertilization, qPCR quantitative real-time polymerase chain reaction, dpf days post fertilization. .

Article Snippet: We purchased Tg(-1.9mylpfa:EGFP) transgenic zebrafish strain (ihb16Tg/+( AB )) from the Shanghai Model Organisms Center.

Techniques: Expressing, Control, Muscles, Standard Deviation, Real-time Polymerase Chain Reaction

Journal: EMBO Molecular Medicine

Article Title: FOXK2 in skeletal muscle development: a new pathogenic gene for congenital myopathy with ptosis

doi: 10.1038/s44321-025-00247-x

Figure Lengend Snippet: ( A ) Gross morphology of Tg(-1.9mylpfa:EGFP) zebrafish larvae at 72 hpf. ( B ) Digital movement trajectories of zebrafish larvae at 5 dpf. One embryo was placed per well ( n = 10 per group). MO morpholino, hpf hours post fertilization, dpf days post fertilization.

Article Snippet: We purchased Tg(-1.9mylpfa:EGFP) transgenic zebrafish strain (ihb16Tg/+( AB )) from the Shanghai Model Organisms Center.

Techniques:

Journal: EMBO Molecular Medicine

Article Title: FOXK2 in skeletal muscle development: a new pathogenic gene for congenital myopathy with ptosis

doi: 10.1038/s44321-025-00247-x

Figure Lengend Snippet: ( A ) Structure of the zebrafish foxk2 gene. The zebrafish foxk2 gene was targeted using specific MOs to inhibit transcription at the start codon (ATG-MO) and proper splicing of exon 3 (I2E3-MO). ( B ) Quantitative of foxk2 expression level by qPCR. Larvae were collected at 72 hpf ( n = 3 repeats). P value: **** P < 0.0001. ( C ) Percentage of larvae exhibiting defect or mortality. The control-MO group included 379 larvae, the ATG-MO group included 296 larvae, and the I2E3-MO group included 375 larvae. ( D ) Survival rate of larvae over a 72-hpf time course in control-MO, ATG-MO, and I2E3-MO groups. ( E ) Gross morphology of Tg(-1.9mylpfa:EGFP) larvae at 72-hpf, along with the quantification of curvature angle of larvae ( n = 10 larvae for each group). The angle formed between the blue arrows indicates the degree of body curvature in the larvae. Scale bars: 500 µm. P value: **** P < 0.0001. ( F ) Body muscle morphology of Tg(-1.9mylpfa:EGFP) larvae at 72-hpf. Blue and yellow arrows denote normal and defective somites, respectively. The white asterisk indicates an abnormal trunk orientation. The inset in the upper right corner shows a magnified view of the boxed area (scale bars: 10 µm for the inset, 50 µm for the full image). ( G ) Cranial facial muscles of Tg(-1.9mylpfa:EGFP) larvae at 72-hpf. The inset in the upper right corner shows a magnified view of the boxed area (scale bars: 40 µm for the inset, 200 µm for the full image). ( H – K ) Statistical analysis of ( H ) movement distance ( P value: **** P < 0.0001), ( I ) velocity (**** P < 0.0001), ( J ) maximum acceleration (**** P < 0.0001) and ( K ) mobility (percentage of actual moving distance to total distance) (*** P = 0.0001; * P = 0.0231) in larvae at 5-dpf ( n = 10 larvae for each froup). Data were analyzed by Student’s t test. All error bars indicate the mean ± standard deviation. MO morpholino, hpf hours post fertilization, qPCR quantitative real-time polymerase chain reaction, dpf days post fertilization. .

Article Snippet: Tg(-1.9mylpfa:EGFP) transgenic zebrafish strain , Shanghai Model Organisms Center , ihb16Tg/+(AB).

Techniques: Expressing, Control, Muscles, Standard Deviation, Real-time Polymerase Chain Reaction

Journal: EMBO Molecular Medicine

Article Title: FOXK2 in skeletal muscle development: a new pathogenic gene for congenital myopathy with ptosis

doi: 10.1038/s44321-025-00247-x

Figure Lengend Snippet: ( A ) Gross morphology of Tg(-1.9mylpfa:EGFP) zebrafish larvae at 72 hpf. ( B ) Digital movement trajectories of zebrafish larvae at 5 dpf. One embryo was placed per well ( n = 10 per group). MO morpholino, hpf hours post fertilization, dpf days post fertilization.

Article Snippet: Tg(-1.9mylpfa:EGFP) transgenic zebrafish strain , Shanghai Model Organisms Center , ihb16Tg/+(AB).

Techniques: